anti ebp2 goat polyclonal Search Results


fluorescein isothiocyanate conjugated donkey anti rabbit antibody  (Santa Cruz Biotechnology)
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anti ebna1  (OriGene)
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secondary horseradishperoxidase conjugated goat anti mouse antibody  (Bio-Rad)
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secondary horseradish peroxidase conjugated goat anti rabbit antibody  (Bio-Rad)
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Bio-Rad secondary horseradish peroxidase conjugated goat anti rabbit antibody
Secondary Horseradish Peroxidase Conjugated Goat Anti Rabbit Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fibrillarin  (Santa Cruz Biotechnology)
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rabbit polyclonal rpa194  (Proteintech)
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pes1  (Proteintech)
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Proteintech pes1
FIGURE 4. Mybbp1a is part of a RNA-sensitive protein complex. A, RNase A-treated and mock-treated (ctrl) HeLa nuclear extracts were separated on a Superose 6 gel filtration column. Load (9%), and collected fractions (every second fraction from 12 to 34) were analyzed by Western blotting with the indicated antibodies. B, nuclear extracts of HeLa cells either transfected with FLAG-Mybbp1a expression plasmid (lane 5–8) or control DNA (ctrl; lane 1–4) were incubated with -FLAG-agarose. Load (L; 5%), flow-through (FT; 5%), bead-bound (B), and eluted proteins (E) were analyzed by Western blotting with the indicated antibodies. C, polysome fractionation was performed on a sucrose gradient, and Mybbp1a and <t>Pes1</t> protein levels of the fractions were detected by immu- noblotting. Pre-assembled ribosomal subunit, monosome, and polysome fractions were detected by measuring UV absorbance at 254 nm as indicated. D, nuclear extracts of HeLa cells either transfected with FLAG-Mybbp1a expression plasmid or control DNA (ctrl) were used for immunoprecipitation with -FLAG-agarose. Eluted protein fractions were separated by SDS-PAGE, silver-stained, and protein bands were cut and subjected to MALDI-TOF mass spec- trometry. Proteins identified are indicated, and their participation in ribosome biogenesis is summarized on the right. hnRNP, heterogeneous nuclear ribonucleoprotein.
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cell line human embryonic kidney 239t hek293t atcc crl 3216 rrid cvcl 0063 chemicals pei polysciences  (Kyfora Bio)
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Kyfora Bio cell line human embryonic kidney 239t hek293t atcc crl 3216 rrid cvcl 0063 chemicals pei polysciences
FIGURE 4. Mybbp1a is part of a RNA-sensitive protein complex. A, RNase A-treated and mock-treated (ctrl) HeLa nuclear extracts were separated on a Superose 6 gel filtration column. Load (9%), and collected fractions (every second fraction from 12 to 34) were analyzed by Western blotting with the indicated antibodies. B, nuclear extracts of HeLa cells either transfected with FLAG-Mybbp1a expression plasmid (lane 5–8) or control DNA (ctrl; lane 1–4) were incubated with -FLAG-agarose. Load (L; 5%), flow-through (FT; 5%), bead-bound (B), and eluted proteins (E) were analyzed by Western blotting with the indicated antibodies. C, polysome fractionation was performed on a sucrose gradient, and Mybbp1a and <t>Pes1</t> protein levels of the fractions were detected by immu- noblotting. Pre-assembled ribosomal subunit, monosome, and polysome fractions were detected by measuring UV absorbance at 254 nm as indicated. D, nuclear extracts of HeLa cells either transfected with FLAG-Mybbp1a expression plasmid or control DNA (ctrl) were used for immunoprecipitation with -FLAG-agarose. Eluted protein fractions were separated by SDS-PAGE, silver-stained, and protein bands were cut and subjected to MALDI-TOF mass spec- trometry. Proteins identified are indicated, and their participation in ribosome biogenesis is summarized on the right. hnRNP, heterogeneous nuclear ribonucleoprotein.
Cell Line Human Embryonic Kidney 239t Hek293t Atcc Crl 3216 Rrid Cvcl 0063 Chemicals Pei Polysciences, supplied by Kyfora Bio, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti ebp2 goat polyclonal  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology anti ebp2 goat polyclonal
FIGURE 4. Mybbp1a is part of a RNA-sensitive protein complex. A, RNase A-treated and mock-treated (ctrl) HeLa nuclear extracts were separated on a Superose 6 gel filtration column. Load (9%), and collected fractions (every second fraction from 12 to 34) were analyzed by Western blotting with the indicated antibodies. B, nuclear extracts of HeLa cells either transfected with FLAG-Mybbp1a expression plasmid (lane 5–8) or control DNA (ctrl; lane 1–4) were incubated with -FLAG-agarose. Load (L; 5%), flow-through (FT; 5%), bead-bound (B), and eluted proteins (E) were analyzed by Western blotting with the indicated antibodies. C, polysome fractionation was performed on a sucrose gradient, and Mybbp1a and <t>Pes1</t> protein levels of the fractions were detected by immu- noblotting. Pre-assembled ribosomal subunit, monosome, and polysome fractions were detected by measuring UV absorbance at 254 nm as indicated. D, nuclear extracts of HeLa cells either transfected with FLAG-Mybbp1a expression plasmid or control DNA (ctrl) were used for immunoprecipitation with -FLAG-agarose. Eluted protein fractions were separated by SDS-PAGE, silver-stained, and protein bands were cut and subjected to MALDI-TOF mass spec- trometry. Proteins identified are indicated, and their participation in ribosome biogenesis is summarized on the right. hnRNP, heterogeneous nuclear ribonucleoprotein.
Anti Ebp2 Goat Polyclonal, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ebp2+goat+polyclonal/IGF-IR%CE%B1+Antibody/pm29941930-517-10-20
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Image Search Results


FIGURE 4. Mybbp1a is part of a RNA-sensitive protein complex. A, RNase A-treated and mock-treated (ctrl) HeLa nuclear extracts were separated on a Superose 6 gel filtration column. Load (9%), and collected fractions (every second fraction from 12 to 34) were analyzed by Western blotting with the indicated antibodies. B, nuclear extracts of HeLa cells either transfected with FLAG-Mybbp1a expression plasmid (lane 5–8) or control DNA (ctrl; lane 1–4) were incubated with -FLAG-agarose. Load (L; 5%), flow-through (FT; 5%), bead-bound (B), and eluted proteins (E) were analyzed by Western blotting with the indicated antibodies. C, polysome fractionation was performed on a sucrose gradient, and Mybbp1a and Pes1 protein levels of the fractions were detected by immu- noblotting. Pre-assembled ribosomal subunit, monosome, and polysome fractions were detected by measuring UV absorbance at 254 nm as indicated. D, nuclear extracts of HeLa cells either transfected with FLAG-Mybbp1a expression plasmid or control DNA (ctrl) were used for immunoprecipitation with -FLAG-agarose. Eluted protein fractions were separated by SDS-PAGE, silver-stained, and protein bands were cut and subjected to MALDI-TOF mass spec- trometry. Proteins identified are indicated, and their participation in ribosome biogenesis is summarized on the right. hnRNP, heterogeneous nuclear ribonucleoprotein.

Journal: Journal of Biological Chemistry

Article Title: Myb-binding Protein 1a (Mybbp1a) Regulates Levels and Processing of Pre-ribosomal RNA

doi: 10.1074/jbc.m111.303719

Figure Lengend Snippet: FIGURE 4. Mybbp1a is part of a RNA-sensitive protein complex. A, RNase A-treated and mock-treated (ctrl) HeLa nuclear extracts were separated on a Superose 6 gel filtration column. Load (9%), and collected fractions (every second fraction from 12 to 34) were analyzed by Western blotting with the indicated antibodies. B, nuclear extracts of HeLa cells either transfected with FLAG-Mybbp1a expression plasmid (lane 5–8) or control DNA (ctrl; lane 1–4) were incubated with -FLAG-agarose. Load (L; 5%), flow-through (FT; 5%), bead-bound (B), and eluted proteins (E) were analyzed by Western blotting with the indicated antibodies. C, polysome fractionation was performed on a sucrose gradient, and Mybbp1a and Pes1 protein levels of the fractions were detected by immu- noblotting. Pre-assembled ribosomal subunit, monosome, and polysome fractions were detected by measuring UV absorbance at 254 nm as indicated. D, nuclear extracts of HeLa cells either transfected with FLAG-Mybbp1a expression plasmid or control DNA (ctrl) were used for immunoprecipitation with -FLAG-agarose. Eluted protein fractions were separated by SDS-PAGE, silver-stained, and protein bands were cut and subjected to MALDI-TOF mass spec- trometry. Proteins identified are indicated, and their participation in ribosome biogenesis is summarized on the right. hnRNP, heterogeneous nuclear ribonucleoprotein.

Article Snippet: Antibodies—The following antibodies were used for Western blotting, ChIP, and immunostaining: rabbit polyclonal -RPA116 (30), rabbit polyclonal -PAF53 (34), and -TIF-IA (Ref. 35; kindly provided by I. Grummt), rabbit polyclonal -Mybbp1a (generated to a GST fusion protein containing amino acid residues 976–1263 of human Mybbp1a) and -mouse Mybbp1a (23), mouse monoclonal -FLAG (M2, Sigma), rabbit polyclonal -FLAG (Sigma), mouse monoclonal -tubulin (Dm1a, Sigma), rabbit polyclonal -RPA194 (sc28174, SantaCruzBiotechnology), -BrdU (RocheApplied Science), -Pes1 (36), -TTF-I (37), -fibrillarin (P2G3 (38)), -B23 (sc-56622, Santa Cruz Biotechnology), -EBP2 (kindly provided by L. Frappier (39)), -rpS2 (kindly provided by M. Bedford (40)), -DDX21 (ProteintechGroup), -Nol1, -GFPAlexa488 (GBA-488, ChromoTek), goat- -mouse-Alexa488 F(ab )2 (Molecular Probes), and goat- -rabbit-Alexa594 (Molecular Probes).

Techniques: Filtration, Western Blot, Transfection, Expressing, Plasmid Preparation, Control, Incubation, Fractionation, Immunoprecipitation, SDS Page, Staining, Mass Spectrometry